Senior Research Associate - San Francisco, California | CareerCircle
Job thumbnail

Senior Research Associate

Actalent

02/22/2022

Posting ID: JP-002664389

×Not Interested
Save Job
Pin drop icon
San Francisco, California
Full Time
Share:Facebook iconTwitter iconLinkedin icon

Description:

About this role:

We are seeking a Research Assistant to join our team to develop new methods for engineering RNA and DNA. This individual will work on projects focused on engineering cell lines, primary cells, and tissues with programmable RNA and DNA targeting systems. The ideal candidate has a strong interest in technology development, experience with diverse technologies and disciplines, and enjoys working in a multidisciplinary environment.

Primary Responsibilities:

• Culture, maintenance, and transfection of cell lines, primary cells, and tissues

• Design and analysis of genome and transcriptome editing experiments

• Purification of DNA and RNA, preparation of libraries for Illumina sequencing

• qPCR, flow cytometry and fluorescence-based microscopy

Qualifications/Skills:

• Experience with molecular cloning, qPCR, RNA techniques, plasmid transfection, flow cytometry and/or fluorescence microscopy

• Experience with maintenance and manipulation of human and/or mouse primary cells or stem cells

• Familiar with scientific software for analyzing and reporting data

• Commitment to rigor in scientific research

• Excellent written and oral communication skills

• Highly collaborative and team-oriented

Education / Experience:

• BS/MS in molecular and cell biology, biochemistry, or a related discipline

• Minimum of 2 years of independent research experience

Preferred Qualifications:

Prior experience with-

• High-throughput assay development and automation

• NGS library preparation, sequencing, and analysis

• CRISPR-based genome editing;

• RNA- or DNA- targeted CRISPR-Cas systems,

• Pooled functional screens with complex libraries (e.g. CRISPRi, CRISPRa, RNAi, shRNA)

• Protein engineering; directed evolution, PACE, or RNA engineering

• Confocal microscopy and/or high content imaging

• Flow cytometry

• Basic command line proficiency (e.g., working knowledge of Linux, Python)

Top Skills Details:

-Molecular Biology

-PCR

-Mammalian Cell Culture

-Flow Cytometry

-Research

-Cell Culture

Additional Skills & Qualifications:

Candidate must have experience in a production style research setting. We have previously subbed a candidate with the skillsets, but the experience was in the context of downstream fermentation processing and the client passed on them. Must walk in the door on the first day with expertise in the top skillsets.

Experience Level:

Expert Level

About Actalent:

Actalent connects passion with purpose. Our scalable talent solutions and services capabilities drive value and results and provide the expertise to help our customers achieve more. Every day, our experts around the globe are making an impact. We’re supporting critical initiatives in engineering and sciences that advance how companies serve the world. Actalent promotes consultant care and engagement through experiences that enable continuous development. Our people are the difference. Actalent is an operating company of Allegis Group, the global leader in talent solutions.

The company is an equal opportunity employer and will consider all applications without regards to race, sex, age, color, religion, national origin, veteran status, disability, sexual orientation, gender identity, genetic information or any characteristic protected by law.

Contact Information

Recruiter: Madeline Alexander

Phone: +16232592828

Email: madalexander@actalentservices.com

Related Courses

Blog